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Configuring FRAP General Options (Full Recovery only)
General FRAP options include settings related to the curve generated based on the FRAP data and bleach position locator (drop intensity threshold). To configure these settings, on the File menu, click FRAP Configurations, and click General.
General options
Curve Fitting Options
The Curve Fitting Options apply to the curve FRAP generates based on the pictures taking during the FRAP process. Under the Curve Fitting Options, you can choose one of two component fittings, Single Component Fitting or Double Component Fitting, explained below.
- Single Component Fitting
The Single Component Fitting fits the pixel intensity recovery data with one Bessel function. The result of this numerical fitting is a single diffusion rate. This method is accurate enough for most applications, if protein molecules are labeled with dye.
- Double Component Fitting
The Double Component Fitting fits the pixel intensity recovery data with two Bessel functions. The result of this numerical fit is two distinct diffusion rates. This method is useful if the protein is not purified well. Some small molecule lipids are labeled with dye, which generate the two distinct diffusion rates: diffusion rate of lipid and diffusion rate of protein.
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